**Laboratory Safety and Infection Control Protocols

This lesson focuses on laboratory safety and quality control, crucial elements for phlebotomists. You will learn about biohazard handling, infection control, proper use of personal protective equipment (PPE), and how to implement quality control measures to ensure accurate test results.

Learning Objectives

  • Identify and describe the key elements of laboratory safety protocols, including the Bloodborne Pathogens Standard.
  • Demonstrate proper donning and doffing of Personal Protective Equipment (PPE).
  • Explain the importance of quality control in phlebotomy and identify pre-analytical error sources.
  • Understand and apply the principles of proper specimen labeling, handling, and storage.

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Lesson Content

Laboratory Safety Protocols

Laboratory safety is paramount in phlebotomy. This includes understanding and adhering to the Occupational Safety and Health Administration (OSHA) Bloodborne Pathogens Standard. This standard mandates practices to protect healthcare workers from exposure to infectious agents. Key elements include:

  • Universal Precautions: Treating all patient specimens as potentially infectious.
  • Hand Hygiene: Frequent and proper handwashing with soap and water or alcohol-based hand sanitizer.
  • Personal Protective Equipment (PPE): Correct use of gloves, gowns, masks, and eye protection to prevent exposure.
  • Safe Work Practices: Proper handling of sharps (needles, lancets) to prevent needlestick injuries, including immediate disposal in sharps containers. Decontamination of work surfaces with appropriate disinfectants (e.g., bleach solutions).
  • Exposure Control Plan: Procedures for handling exposures, including reporting and post-exposure prophylaxis.

Example: Imagine a scenario where you're drawing blood from a patient. Before starting, you ALWAYS put on gloves. After the draw, you immediately dispose of the needle in a sharps container and remove your gloves, washing your hands thoroughly. Any spills of blood or body fluids are immediately cleaned and disinfected.

Personal Protective Equipment (PPE)

Proper use of PPE is essential. This includes:

  • Gloves: Must be worn for every blood draw and changed between patients. Inspect gloves for tears before use.
  • Gowns: Protect clothing and skin from splashes.
  • Masks/Face Shields: Used to protect against splashes or sprays of blood or body fluids.
  • Eye Protection: Goggles or face shields protect the eyes.

Donning PPE sequence (putting on): 1. Hand hygiene. 2. Gown. 3. Mask. 4. Eye protection. 5. Gloves.
Doffing PPE sequence (taking off): 1. Gloves (handle outside of gloves only). 2. Hand hygiene. 3. Eye protection. 4. Gown. 5. Mask. 6. Hand hygiene.

Example: You are preparing to collect a blood specimen from a patient with suspected influenza. You put on a gown, a face mask, and then eye protection, before donning gloves.

Infection Control

Infection control involves practices to prevent the spread of infectious diseases. This includes:

  • Hand Hygiene: The single most effective measure.
  • Proper Waste Disposal: Biohazardous waste (e.g., used needles, blood-soaked materials) must be disposed of in designated containers, usually red bags. Regular waste is disposed in regular trash.
  • Surface Disinfection: Regularly cleaning and disinfecting work surfaces.
  • Isolation Precautions: Adhering to standard and transmission-based precautions as required.

Example: After performing a venipuncture, you immediately dispose of the needle in a sharps container. You then remove your gloves, wash your hands, and clean and disinfect your work area.

Quality Control in Phlebotomy

Quality control (QC) ensures accurate and reliable test results. This is achieved by:

  • Pre-analytical Variables: Identify and control variables that can occur BEFORE the sample is analyzed, affecting results: Patient identification, incorrect tube type, improper order of draw, incomplete filling of tubes, delays in transport/processing, hemoconcentration due to prolonged tourniquet use.
  • Specimen Handling: Proper labeling with patient information, date, time of collection, and your initials. Transport specimens according to laboratory protocols (e.g., temperature requirements, within a specific timeframe).
  • Post-analytical Variables: Review and understanding of laboratory results, flagging abnormal results, and communication with the healthcare provider.

Example: Before drawing blood, you confirm the patient's identity using two identifiers (name and date of birth). You select the correct tube type according to the test order and ensure the tube is filled adequately. After collection, you label the tube immediately and transport it promptly to the lab.

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